Microbial profile of root canals of pulpally infected teeth in Ghanaians

Introduction: Pulpal and periapical infections are initiated by microorganisms when they gain access to the dental pulp. The success of root canal treatment principally depends on eradication micro-organisms in system. aim this study was evaluate viable microbial profile canals with various stages infection Ghanaians. Material Methods: Forty-four consecutive patients sixty teeth referred Restorative Dentistry Clinic requiring were recruited. Root samples collected from sterile paper points. processed subjected analysis identification using Matrix-assisted laser desorption/ionization-time flight (MALDI-TOF) mass spectrometry (MS). Results: A total 259 isolates recovered 60 infected canals, belonging twenty different genera. Out species isolated, only two Candida albicans, a fungi; 257 (99.2%) bacterial 19 genera encompassed 53 species, out which 26 (49.1%) identified as facultative anaerobes, 15 (28.3 %.) obligate anaerobes 12 (22.6%) aerobes. Streptococcus (Streptococcus oralis, mitis, mutans constellatus) most predominant isolates, followed Prevotella sp, Actinomyces Enterococcus faecalis Rothia sp respectively. Conclusion: findings show that polymicrobial nature. determination aids understanding pathogenesis pulpal periradicular helps choosing effective antimicrobial irrigation medicament for treatment.